Primer DB TM

A Validated PCR Primer Database for Microorganisms
  Organism Input

Choose organism:
Bacteria:
Virus:
Fungi:
NIH Categorized Agents:
CDC Categorized Agents:
Choose locating method:
GenBank Annotation
TIGR Glimmer v2.13 Glimmer options:
Reference CDS by:
Number CDS:
Gene Name (Annotation only)
Locus Tag (Annotation only)

Retrieve validated primers from database



  Validated Primer Input

Edit primer sequences for submission.

Enter forward primer sequence(s):
 
Enter reverse primer sequence(s):



  Validated Primer Picking Parameters

Edit primer picking parameters for submission.

No.to Return: Max 3' Stability:
Max Mispriming: Pair Max Mispriming:
Product Size Min: Opt: Max:
Primer Size Min: Opt: Max:
Primer Tm Min: Opt: Max:
Primer GC% Min: Opt: Max:
Max Self Complementarity: Max 3' Self Complementarity:
Max #N's: Max Poly-X:
CG Clamp: Max Tm Difference:
Generate Internal Primers Generate RT-PCR primers


  Sequence Options

Edit sequence parameters for submission.

Split CDS > 500 bp

Flanking sequence for CDS: nucleotides

Buffer sequence between primer and CDS: nucleotides


  Contact Information

Contact information is REQUIRED for submission.

First name:      Last name:      Email address:

Submit validated primers to database

Primer DB has been run [3,199] times

Please email errors or comments to PathoGene Tech Support


A service of the RCE Region VI Computational Biology Group. Email errors or comments to PathoGene Tech Support
Copyright © 2004 UT Southwestern Medical Center and RCE Region VI Computational Biology Group. All rights reserved.