PCR Now TM

A Real-Time PCR Primer Design Tool
  Input

Enter any number of sequences in FASTA format:
or upload a FASTA format file (PLAIN-TEXT only):

These symbols should NOT be used in the comments field: ` # $ % & ( ) \ | ; ' " < > / ?




  Primer Picking Parameters

No.to Return: Max 3' Stability:
Max Mispriming: Pair Max Mispriming:
Product Size Min: Opt: Max:
Primer Size Min: Opt: Max:
Primer Tm Min: Opt: Max:
Primer GC% Min: Opt: Max:
Max Self Complementarity: Max 3' Self Complementarity:
Max #N's: Max Poly-X:
CG Clamp: Max Tm Difference:


  Email Option

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A service of the RCE Region VI Computational Biology Group. Email errors or comments to PCR Now Tech Support
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